Capel-205 is a new generation capillary electrophoresis system with advanced options and design. Its main features are:
Extended autosampler capacity up to 59 positions, allowing to provide overnight analyses
Standard centrifuge-type vials of 1.5mL volume (Eppendorf-type) closed with their original caps are used for all solutions
Specially designed unit automatically opens vials just prior to their use, thus preventing evaporation and any kinds of cross-contamination
New HV block provides voltage up to +/- 30 kV thus improving separation efficiency and decreasing analysis time
Optionally – integrated unit to control the state of the inner capillary surface provides significantly improved migration time reproducibility especially when analyzing samples with complex matrices
Thus Capel-205 can be the very best choice not only for R&D and low-scale analyses but also for the customers dealing routinely with dozens of samples per day.
PRINCIPLE OF OPERATION
High performance capillary electrophoresis is based on the differential migration of components of aqueous samples within a narrow fused silica capillary driven by an electric field. Separated solutes are quantitatively detected at the capillary outlet by a high sensitive optical system based on direct or indirect UV absorbance.
FEATURES AND BENEFITS
Extended instrumental options
Extended autosampler capacity up to 59 positions – hundreds of samples per months can be analyzed
Integrated vials caps removal – all solutions are kept in the autosampler tightly closed excluding evaporation and cross-contamination
New HV block provides voltage up to +/- 30 kV – separation efficiency is improved and analysis time is decreased
Redesigned capillary cassette – length of the thermostated part of the capillary is increased to improve migration time reproducibility
Spectra scanning facilitates peak identification
Broad range of controlled injection pressure allows analysis of viscous samples
Reverse sample injection under vacuum: ultra-short analysis time (less than 1 min) and sample stacking to decrease detection limit
Precise and highly effective capillary thermostating provided by circulating liquid coolant (water)(± 0.1 C)
Extended range of applied buffers and increased efficiency in separation
Powerful software package "Elforun"
Modified Elforun-205 to become even more user-friendly when operating with extended autosampler capacity
Increased flexibility in performing analyses of various complexity
Any kinds of complex runs are possible including those with pre-programming of changes in analysis conditions
Customized report, data export to other programs
Unique design of the capillary cassettes
Enlarged thermostatic part of the capillary improves migration time reproducibility
Easy capillary change in just a few minutes
Lower Detection Limit due to the optimized optical scheme
ANALYTICAL CHARACTERISTICS
Detectors CAPEL-205 is equipped with a spectrophotometric detector with a wavelength range of 190 - 400 nm.
Light source
Deuterium lamp
Vials
Standard centrifuge-type (e.g.Eppendorf-type)1.5 ml, minimal sample volume 100 µl.
Injection modes
Sample injections by pressure, vacuum or electrokinetically.
Controlled pressure profile of injection with pressure values from – 100 mbar to + 100 mbar.
Electrokinetic injection at (+/-) 1 – 30 kV considering time for voltage ramping.
Programmable injection time / pressure / voltage.
Analysis
With a Voltage from -30 kV to +30 kV with a 1 kV step and an electronic polarity switch.
Current from 0 to 300 µA.
Elforun software features
Combination of instrument controlling, data acquisition and data processing;
Real time electropherogram visualization;
Electropherogram data processing with overlay mode and basic mathematical operations;
Computation of electrophoresis system parameters;
Customized report output (hard copy and file), data exchanges with worksheets, databases and word processors;
Extensive data processing algorithm customization;
GLP and GALP compliance.
EQUIPMENT AND OPTIONS
Capillary electrophoresis system CAPEL-205;
Spare capillary cassettes;
Elforun software package;
Kits for analyses (by request); most of CE-kits of any manufacturers are compatible with Capel-205.
Warranty
All Lumex instruments and equipment are covered by a 12-months warranty.
Detector
Spectrophotometer
190-400 nm
Analysis
Constant voltage, from –30 kV to +30 kV in 1 kV steps
Automatic (electronic) polarity switch
Current 0 - 300 mkA
Pressure up to 100 mbar
Programmable changing of wavelength, pressure and voltage during analysis
Injection
By voltage, from –30 kV to +30 kV in 1 kV steps;
By pressure, from –100 to 100 mbar in 1 mbar steps
Sampler
Autosamplers for 59 vials (standard Eppendorf-type 1.5 mL)
Rinsing
By pressure, 500–2000 mbar in 1 mbar steps
Capillary
Length: 30–120 cm
Internal diameter: 50, 75 μm
Capillary thermostating
Liquid thermostating, from -10 up to+ 30 °C from ambient temperature, ±0.1°C
