This course introduces the fundamentally important concepts associated with High Performance Liquid Chromatography (HPLC) analysis including hardware basics, modes of analysis, basic troubleshooting, column chemistry, important parameters of the mobile phase such as amount and type of organic solvents, pH, buffer strength and type, and much more. Suitable as a refresher for the more experienced analytical chemist or as an invaluable introduction to the technique for those with limited experience, this course provides a priceless insight into HPLC principles and practice. This course is not instrument-specific.
Who is this course for
Users of LC in any of its modes (NP, RP, HILIC, IEX, SEC…) or anyone interested in gaining a deeper understanding of the chromatographic separation of compounds amenable to analysis by LC.
Previous knowledge
No previous experience of chromatography is necessary, but a basic knowledge of chemistry is beneficial.
What you will learn
How the chromatographic process takes place
The essential theory behind chromatographic separations
The architecture and working principles of a modern HPLC system
The information contained in a chromatogram and the basic chromatographic parameters
The essential principles of sample preparation, mobile phases, buffers and eluent additives for HPLC
